THE ESTIMATION METHOD OF S-ACID LACTIC IN THE FERMENTATION PRODUCTS OF ALOE VERA GEL BY HIGH- PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

 An accurate, precise, simple and economical High Performance Liquid Chromatographic method for the estimation of S-acid lactic in the process of fermentation of Aloe Vera gel has been developed. The method is using HIQSIL 100 C18column (Length: 250nm, Diameter: 4.6nm, Particle size: 5μ) with potassium dihydrogen phosphate as mobile phase. The separation was performed by UV detector at 210 nm wavelength. The flow rate was kept at 1mL/min and the injection volume was 20μl. The results obtained showed that the linearity of the method was 0-180 ppm, and the correlation coefficient was found to be 0.999. Detection limit of the method for S-acid lactic was 1.15 ppm. The mean recoveries were from 95% to 99%. The reproducibility RSDs ranged from 2.87%. The results demonstrated the suitability of the HPLC approach for the analysis of S-acid lactic in the process of fermentation from Aloe Vera gel.