Effects of Caffeine on In Vitro Fertilization of Pig Follicular Oocytes

  • NGUYEN THỊ THUONG
  • NGUYEN TIEN DAT
  • NGUYEN VAN HANH
  • NGUYEN HUU DUC
  • NGUYEN VIET LINH

Abstract

     In the present study, experiments were conducted to assess the effects of caffeine on in vitro fertilization of pig follicular oocytes. Cumulus-oocyte complexes (COCs) were collected from porcine ovaries from slaughterhouses, cultured in in vitro maturation medium 1 (IVM1) at 38.5oC for 20-22 hours and then in in vitro maturation medium 2 (IVM2) for the next 24 hours. Only the oocytes with expanded cumulus cells were selected for in vitro fertilization. Boar frozen semen was used for the porcine IVF procedure. The spermatozoa were pre-incubated in modified TCM 199 medium and subsequently incubated for 3 hours in porcine fertilization medium (pig FM) supplemented with either 2 mM or 5 mM of caffeine. They were cultured in IVC1 medium supplemented with pyruvate and lactate from day 0 to day 2, and then in IVC2 medium supplemented with glucose from day 2 to day 6. The results indicate that pig FM containing 5 mM caffeine gave a higher penetration rate than 2 mM caffeine (33.4% vs. 11.4%, respectively). The blastocyst rates of the two groups were not significantly different (8.4% and 8.0%). In conclusion, a higher caffeine concentration in the fertilization medium may ensure the production of in vitro porcine embryos with acceptable productivity utilizable for further studies on this subject.
điểm /   đánh giá
Published
2019-09-30
Section
ENGINEERING AND TECHNOLOGY