In silico analysis of Hypermethylation in cpgislands of uchl1 gene’s promoter in nasopharyngeal carcino

Abstract

    Background and Objective: The methylation of Ubiquitin C-Terminal Hydrolase L1 (UCHL1) gene has beenreported in many human cancers including nasopharyngeal carcinoma (NPC). In Vietnam, the methylation ofUCHL1 gene's promoter in NPC has not been demonstrated yet. In this study, a systematic literature revision wascarried out to summarize the current evidences about the frequencies of UCHL-1 gene's promoter methylation inNPC for further application in Vietnamese population.Methods: A systematic literature analysis was conducted based on the comprehensive studies. Moreover,many bioinformatic tools such as Methprimer, TFsearch, IDT OligoAnalyzer 3.1 were used to predict the CpGislands, transcriptional factors, and to pick up the MSP (Methylation-Specific PCR) primers.Results: Total of three previous studies were summarized and accessed for eligibility from literature research.As the results, the average weight methylated frequencies were 72.4% and 13.0% for NPC and non-canceroussamples, respectively. The significant association between UCHL-1 promoter methylation and NPC with the OR of10.459 (95% CI = 4.915 - 22.254, p < 0.001) and RR of 4.117 (95% CI = 1.958 - 6.645, p < 0.0001) based on therandom effects model, was observed. Moreover, we were successful in predicting the CpG islands as well asidentifying transcriptional factor binding sites which served as "hot spot" for ideal primer pick up and located ingene promoter.Conclusion: The methylation of UCHL-1 gene promoter was significantly associated and contributed to NPCdevelopmentin which it could be further applied in evaluation of UCHL-1 gene promoter status in Vietnamesepopulation.