STUDY ON BETA- HCG DETERMINATION METHOD USING MAGNETIC NANOPARTICLES

Abstract

Different parameters for using modified mangetite nanoparticles in beta-hCG analysis were studied in order to figure out optimal experimental conditions under which a nonlinear regression calibration equation was obtained. Via fluorescence immunoassay method, LOD and LOQ values were determined in this study as 3,44 and 14,2 mg/L. The experiment results showed that the combination of magnetic nanoparticles to the fluorescence immunoassay method allowed detecting beta-hCG at very low concentration with high precision and accuracy (2.87%). Several authetic blood samples of pregnant women were also analyzed using the studied method, which gave results in a good agreement with the one obtained by using standard kit (CV values under 10%).