SIMULTANEOUS DETERMINATION OF QUINOLONES, SUFONAMIDES AND TRIMETHOPRIM IN SEDIMENT BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC/MS/MS)

Abstract

A sensitive, simple and reliable multi-residue method was developed for the
determination of 9 antibiotics including 4 quinolones, 4 sulfonamides and
trimethoprim in sediment using liquid chromatography–tandem mass
spectrometry (LC/MS/MS). Under optimal conditions: Extraction of antibiotics
from the sediment was carried cut with CH3OH:citrate buffer (1: 1, v/v; pH = 4);
Recoveries were of all target 73.8 - 113.4% with relative standard deviation
(RSD) below 8.4% for two concentrations; The method detection limit (MDL) and
method quantification limit (MQL) of 9 antibiotics ranged 0.02 - 0.21μg/kg and
0.06 - 0.64μg/kg, respectively. The developed method was successfully applied
to the analysis of antibiotics in sediment of West lake and Truc Bach lake.