Cordyceps militaris - Effect of coordination nutritional ingredients on the development and cordycepin, adenosin production of Cordyceps militaris

DOI: 10.18173/2354-1059.2021-0071

Abstract

Cordyceps militaris is an insect parasitic fungus with high medicinal value similar to Cordyceps sinensis and has great economic value. Ensuring the balance between the biomass and the bioactive potential of C. militaris plays an important role in the development of quality products from this medicinal fungus. In this study, nitrogen sources (fresh silkworm pupae, peptone, yeast extract) with different concentrations were used to evaluate the effect on yield, morphology and content of cordycepin, adenosine of C. militaris. The results showed that the N1 medium using 20 g\L fresh silkworm pupae, 0.5g\L KH2PO4 and 0.5g\L MgSO4 gave the highest yield and active ingredient content, with cordycepin yield of 17.01 mg\jar. Adding carbon sources (glucose and sucrose) at concentrations (10, 20, 40 g\L) to N1 medium can shorten the time of filamentous spreading and the time of fruit body formation, but significantly reduce cordycepin content. This result has important implications for medium optimization for C. militaris culturing.