Highly pure alpha-hederin from Hedera helix leaves isolated by centrifugal partition chromatography

Tóm tắt

Introduction: Hedera helix is a species of the Araliaceae family, which contains flavonoids, coumarins, phenolic acids, sterols, alkaloids, anthocyanins, and triterpene saponins. α-Hederin is a saponin compound of the H. helix that possesses many potential biological activities. In previous studies, several methods were used to isolate and purify α-hederin from ivy leaves. However, the loss of α-hederin obtained during solid chromatography purification was relatively high, and it was difficult to obtain α-hederin with high purity. In this research, a centrifugal partition chromatography (CPC) method was established for the isolation of α-hederin directly from ethanol crude extract of Hedera helix leaves from Da Lat, Lam Dong province, Vietnam.

Methods: The separation of α-hederin was carried out by applying centrifugal partition chromatography with a PLC 2250 system (Gilson Inc., France) coupled with an SCPC-250 column (Armen Instrument, France). In a CPC run, a two-phase solvent system consisting of n-hexane–ethyl acetate–methanol–water (2:3:2:3, v/v) in ascending mode (the lower phase was the stationary phase), with a rotation speed of 1600 rpm and a flow rate of 8.0 mL/min, was used.

Results: The purity of the isolated α-hederin was 95.7%, as confirmed by HPLC-DAD at 210 nm. The structure of purified α-hederin was determined by 1H-NMR and 13C-NMR. The yield of α-hederin was 12.7%.

Conclusion: This investigation might be used as a reference for the large-scale separation of high-purity α-hederin from H. helix leaves.