Preliminary study on preservation of recombinant HG-CSF by lyophilization

Abstract

hG-CSF is a cytokine that stimulates the proliferation, differentiation, function of mature neutrophils and is generally used for treatment of neutropenia in cancer patients under chemotherapy or other diseased patients. After production process, E. coli-derived recombinant hG-CSF is usually available as non-glycosylated, liquid protein, so that it’s often unstable, easy to lose their biological activity. In this study, we report the results of using lyophilization to storage recombinant hG-CSF. We found out the freezing conditions and the suitable protein volumes for freeze-drying process.  Furthermore, we also studied the effects of lyophilization on this protein by testing the structure with Native-PAGE, RP-HPLC, testing biological activity on M-NFS-60 cell lines and in mouse (Mus musculus).