Tạp chí Phân tích Hóa, Lý và Sinh học

PHÁT TRIỂN PHƯƠNG PHÁP SẮC KÝ LỎNG KHỐI PHỔ HAI LẦN XÁC ĐỊNH HÀM LƯỢNG MỘT SỐ HỢP CHẤT PER- VÀ POLYFLUOROALKYL (PFAS) TRONG THỰC PHẨM

Lưu Thị Huyền Trang — 2025-09-26

PFAS are a large, complex group of synthetic chemicals that are highly resistant to degradation in the
environment. They may cause cancer and adverse effects on the reproductive and immune systems. In this
study, 16 PFAS compounds were determined in food samples (fish, meat, vegetables) using the LC-MS/MS
method to assess contamination levels and support food safety risk control. A reversed-phase C18
chromatography column (150 mm × 2.1 mm; ID 3.5 µm) was used to separate the 16 compounds. The
mobile phase consisted of (A) 2 mM ammonium acetate (CH3COONH4 )/methanol (MeOH) (9/1, v/v) and
(B) MeOH. The extraction solvent was 1% formic acid (HCOOH)/acetonitrile (ACN), and the extraction salt
compositions contained 6 g MgSO₄ combined with 2 g NaCl. Samples were cleaned up by d-SPE
composition of 1.2 g MgSO₄ combined with 0.2 g PSA and 0.2 g C18, and SPE using Polymeric Weak Anion
SPE cartridge. The calibration curves for the 16 substances, within a concentration range of 0.1–5.0 µg/kg,
showed correlation coefficients (R²) greater than 0.995. The method achieved recoveries ranging from
75.7% to 110%, with relative standard deviations (RSDs) between 1.46% and 9.72%. The method detection
limit (MDL) and method quantitation limit (MQL) were 0.03 µg/kg and 0.1 µg/kg, respectively. This method
was applied to the analysis of 20 food samples, and the results showed that 5 of these samples tested positive
for specific PFAS compounds. This study may lay the foundation for developing quantitative procedures for
PFAS groups in various food matrices.

NGHIÊN CỨU XÁC ĐỊNH HÀM LƯỢNG MỘT SỐ KIM LOẠI NẶNG TRONG HƯƠNG NHU TÍA VÙNG BẮC TRUNG BỘ BẰNG PHƯƠNG PHÁP QUANG PHỔ HẤP THỤ NGUYÊN TỬ (AAS)

Đậu Đăng Thiện — 2025-09-26

The content of some heavy metals such as cadmium, copper, iron, manganese, nickel, lead, and zinc (Cd,
Cu, Fe, Mn, Ni, Pb, Zn) in Ocimum Tenuiflorum grown in the two provinces of Nghe An and Ha Tinh in the
North Central region of Vietnam has been determined using atomic absorption spectroscopy with flame
atomization (F-AAS) after sample digestion in microwave oven. The analysis results were compared with the
agricultural crop guidelines of FAO/WHO, showing that the heavy metal content was within the limits. The
analysis results were also compared with some other studies on Ocimum Tenuiflorum finding that the
content of Cu, Fe, Mn, Zn was higher, while the content of Cd, Ni, Pb was lower. According to WHO
standards on medicinal herbal ingredients, the content of Cd, Cu, Mn, Pb below the permissible limits in all
samples; the content of Fe in HN1, HN3, HN5 samples exceeded the recommended value; the content of Zn
in the HN4 sample exceeded the permissible limits. The concentration range of heavy metals varies, with the
levels of toxic metals such as Cd, Pb, and Ni all below the detection limit for all samples. For other heavy
metals, the content of Fe and Mn in sample HN1 (191.606; 96.429 mg/kg); the content of Cu and Zn in
sample HN4 (16.597; 133.561 mg/kg) is the highest. The analysis method used has a low detection limit,
achieving thresholds lower than 59 ppb for Cd, Fe, Mn, Ni, Pb, Zn and 0.1 ppm for Cu. The relative
standard deviation (% RSD) of 10 repeat measurements of low-concentration heavy metal content is all less
than 3.530%; and the recovery of elements are in the range from 87.652 to 109.812%, which meets AOAC
requirements for trace.

ĐÁNH GIÁ PHƯƠNG PHÁP CHIẾT XANH SULFAMETHOXAZOLE TỪ ĐẤT KẾT HỢP SIÊU ÂM VÀ PHÂN TÍCH LC-MS

Nguyễn Tiến Đạt — 2025-09-26

This study aimed to optimize the extraction conditions for sulfamethoxazole (SMX) from soil matrices using
green ultrasound-assisted extraction combined with LC-MS analysis. Two buffer systems, phosphate and
Mcllvaine, at different concentrations (1 mM and 10 mM) and pH values ranging from 3 to 7 were
evaluated. A three-way ANOVA revealed that soil type, buffer type, and pH significantly influenced SMX
recovery, with notable interactions among these factors (p < 0.001). Both phosphate and Mcllvaine buffers
at 1 mM concentration and pH 3 provided the highest and most stable SMX recovery (>100%) across two
contrasting soils (S1 and S2). Conditions of low ionic strength and acidic pH significantly enhanced the
desorption efficiency of sulfamethoxazole (SMX). In contrast, increasing the buffer concentration –
especially when using Mcllvaine buffer—resulted in reduced recovery rates, likely due to the formation of
metal-ligand complexes and ion suppression effects. The findings highlight phosphate 1 mM and Mcllvaine 1
mM at pH 3 as optimal green extraction solutions for SMX analysis in diverse soil environments, providing a
reliable and environmentally friendly approach for monitoring antibiotic residues in agricultural soils.

NGHIÊN CỨU ĐỊNH LƯỢNG β-ECDYSTERON VÀ THÀNH PHẦN HOÁ HỌC TỪ RỄ NGƯU TẤT – ACHYRANTHES BIDENTATA BLUME THU TẠI TỈNH HƯNG YÊN

Nguyễn Thi Duyên — 2025-09-26

In this study, we have developed a quantitative method β-ecdysterone in root samples of Achyranthes
bidentata Blume colleted in Hungyen. Only a sample (NT03) had β-ecdysterone content of 0.051 ± 0.001 %,
while the others were below 0.03%. Four compounds including acutoside A (1), calendoloside E (2), β
ecdysterone (3) and daucosterol (4) were isolated from NT03 sample. Their chemical structures were
determined by MS, 1D-, 2D-NMR spectral methods, which were compared with the data reported in
previous literature.

MỘT SỐ HỢP CHẤT PHÂN LẬP TỪ CỎ LÁ HẸ (HALODULE UNINERVIS)

Hồ Xuân Thuỷ — 2025-09-26

From the methanol extract of the seagrass Halodule uninervis, five pure compounds were isolated for the
first time from this species using a combination of various chromatographic techniques. The chemical
structures of these compounds were elucidated based on the analysis of mass spectrometry data (ESI-MS)
and nuclear magnetic resonance spectroscopy (¹H-NMR, ¹³C-NMR, DEPT, HMBC, HSQC, and COSY), in
conjunction with comparisons to previously published literature. The identified compounds include 3,3’,4’
tri-O-methylellagic acid, catechin, naringenin-4’,7-dimethyl ether, quercetin and quercetin-3-O-rutinoside

NGHIÊN CỨU TÁCH VÀ TINH SẠCH ABRIN TỪ HẠT CAM THẢO DÂY TẠI VIỆT NAM

Ngô Ngọc Trung — 2025-09-26

Abrin is a natural lectin found in the seeds of Abrus precatorius. The structure of abrin consists of two
chains (Chain A and chain B) with a molecular weight about 65 kDa. Abrin is highly toxic to humans and
animals, so it is considered as a dangerous biological agent that can be used in terrorism or biological
warfare. This article introduces the process of isolating abrin from the seeds of Abrus precatorius and
purifying abrin by ion exchange chromatography and gel filtration chromatography. Purified abrin is used
in determining toxicity in animals, testing the sensitivity of rapid detection tests and immunological studies.

NGHIÊN CỨU BAN ĐẦU VỀ THÀNH PHẦN HÓA HỌC VÀ HỌAT TÍNH SINH HÓA CÂY VÔNG VANG (Abelmoschus moschatus) TẠI THÁI NGUYÊN

Trương Thị Thảo — 2025-09-26

Vong vang (Abelmoschus moschatus) is a medicinal plant that has been widely used in Vietnamese folk
medicine. This study presents some initial preliminary research results on the chemical composition and
antibacterial ability of stem parts, leaves, and roots of Vong vang plants collected in Dai Tu, Thai Nguyen. Vong
Vang leaf extract exhibits better antibacterial activity than stem and root extract against all three bacteria:
Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. Vong vang leaf extract shows good
ability to inhibit the proliferation of gastric cancer (AGS) and liver cancer (HepG2) cells, IC50 were 27  3 and 35
 2 µg/mL, respectively. At concentration 100 µg/mL, the effectiveness of inhibiting AGS and HepG2 cancer cell
proliferation is 86.25  0.59 % and 87.30  1.44 %, respectively. These results are a reliable scientific basis for
folk remedies as well as the potential for application in the medical field of the Abelmoschus moschatus plant.

KHẢO SÁT CÁC ĐIỀU KIỆN HÌNH THÀNH NHA BÀO Geobacillus stearothermophilus ATCC 7953

Đặng Thị Thùy Dương min — 2025-09-26

Biological indicators are considered the gold standard for sterilization process control, one of the criteria
for evaluating autoclaves, and a measure that directly reflects the effectiveness of sterilization. In Vietnam,
the use of biological indicators to proactively control the effectiveness of the sterilization process has not
received due attention and has not been implemented regularly, especially in local medical facilities.
Research on Geobacillus stearothermophilus bacteria that create biological indicators is still very limited.
This study investigated 16 conditions for the spores formation of G. stearothermophilus ATCC 7953 from 4
culture mediums NA-CaKMgMn, NA-CaMn, YMMn, PCA; 2 pH conditions of 7.0 and 8.5; 2 incubation
temperatures of 55oC and 61oC. The research results showed that at pH 8.5 and incubation temperature of
61oC, the rate and proportion of G. stearothermophilus ATCC 7953 spores formation were superior to those
at pH 7.0 and 55oC. The four spore formation conditions NA-CaKMnMg/ 8.5/ 61oC, NA-CaMn/ 8.5/ 61oC,
YMMn/ 7.0/ 61oC and YMMn/ 8.5/ 61oC had a spore formation rate of over 95% and a spore yield of over
105 cfu/mL. Of which, the condition YMMn/8.5/ 61oC had the fastest rate of G. stearothermophilus ATCC
7953 spores formation, reaching 90% after 4 days. The results of the research could be the premise for
further research to create biological indicators that meet ISO standards.

KHẢO SÁT THÀNH PHẦN HÓA HỌC VÀ HOẠT TÍNH CỦA TINH DẦU LÁ BẠCH ĐÀN (EUCALYPTUS GLOBULUS) VÀ LÁ BẠCH ĐÀN CHANH (EUCALYPTUS CITRIODORA) THU HÁI TẠI TỈNH ĐỒNG THÁP

Đặng Xuân Dự — 2025-09-26

Cây bạch đàn Eucalyptus globulus và cây bạch đàn chanh Eucalyptus citriodora rất phổ biến ở Việt Nam và
nhiều quốc gia trên thế giới. Tinh dầu của 2 loài này được sử dụng rộng rãi trong đời sống hàng ngày với
nhiều công dụng hữu ích bảo vệ sức khỏe như sát khuẩn, kháng viêm, bảo vệ da, trị mụn, giảm stress,…
Mặc dù là 2 loài cùng chi với nhiều đặc điểm tương đồng về cảm quan và công dụng, tuy nhiên kết quả phân
tích GC-MS cho thấy chúng hầu như khác biệt về thành phần đơn hương, trong đó thành phần chính của tinh
dầu lá E. globulus là α–phellandrene (36.9%), cymol (34.2%) trong khi thành phần chính của tinh dầu lá E.
citriodora là citronellal (67.54%), isopulegol (10.09% ), (R)–(+)–β–citronellol (9.9%). Cả 2 loại tinh dầu
đều thể hiện hoạt tính kháng oxy hóa yếu đối với gốc tự do DPPH và ABTS•+ trong khi hoạt tính kháng
khuẩn tốt trên 5 dòng vi khuẩn Bacillus cereus, Bacillus subtilis,Staphylococcus aureus, Pseudomonas
aeruginosa và Escherichia coli.