HOẠT TÍNH GÂY ĐỘC TẾ BÀO VÀ ỨC CHẾ MIỄN DỊCH CỦA CHẤT METHYL CAFFEATE PHÂN LẬP TỪ LOÀI NGỌC CẨU (BALANOPHORA LAXIFLORA)

  • Ba Thi Cham
  • Nguyen Thi Thuy Linh
  • Nguyen Thi Hoang Anh
  • Nguyen Phi Hung
  • Do Thi Thao
  • Nguyen Quyet Tien
  • Nguyen Thanh Tam
  • Tran Duc Dai
  • Dang Ngoc Quang
  • Nguyen Thi Thu Hoa
  • Tran Khac Vu
  • Le Thi Hong Nhung
  • Domenico V Delfino
  • Trinh Thi Thuy
Keywords: Balanophora laxiflora, methyl caffeate, cytotoxicity, RAW264.7, interleukin, immunosuppressive effect

Abstract

Methyl caffeate, a major component, was isolated by column chromatography from Balanophora laxiflora growing in Tuyen Quang. The cytotoxic activity of
methyl caffeate has been evaluated using A549, T24, Huh-7, 8505c and SNU-1 cell lines. Results showed that methyl caffeate exhibited cytotoxic activity against all
five tested human cancer cell lines (A549, T24, Huh-7, 8505 and SNU-1) with the IC50 values ranging from 28.83g/mL to 50.19g/mL. In addition, the
immunosuppressive capacity of methyl caffeate through inhibition of IL-2 expression in macrophage cells (RAW264.7) was assessed for the first time. The results
exhibited that methyl caffeate had a strong inhibitory effect on IL-2 cytokine secretion on RAW264.7 cells at a concentration of 100µg/mL (P < 0.01). These results
suggest that methyl caffeate could possess immunosuppressive effects in vitro.

điểm /   đánh giá
Published
2022-02-10
Issue
Vol. 57 No. 6 (2021)
Section
RESEARCH AND DEVELOPMENT