OPTIMIZING CONDITIONS FOR SAPONIN ENZYMEASSISTED EXTRACTION FROM Ficus auriculata FRUIT

Abstract

This study aimed to find the optimal conditions for enzyme-assisted extraction (EAE) of saponin from Ficus auriculata. The investigated parameters included enzyme concentrations (0.2; 0.4; 0.6; 0.8; 1%), pH (4; 4.5; 5; 5.5; 6), and temperatures (40; 50; 60; 70; 80 oC). The extraction efficiency was shown by the saponin content. Optimized extraction conditions such as enzyme concentration, pH, and temperature were obtained by the response surface method (RSM). The experimental results at optimal conditions were 0.65%; pH 5.55; at 49.78 oC and the highest saponin content was 38.23 ± 0.98 mg/g dry matter. The finding indicated that EAE was an effective method to extract saponin from F. auriculata.