INVESTIGATION OF SOME FACTORS AFFECTING THE EXTRACTION OF POLYPHENOL AND FLAVONOID COMPOUNDS FROM Ruellia tuberosa L.

Abstract

Ruellia tuberosa, a tropical perennial plant, belongs to the family Acanthaceae and contains many bioactive compounds such as phenolics, flavonoids, triterpenoids, lignans, sterols, etc... Almost all parts of the plant can be used to benefit human health. This study investigated the source of raw materials, the effect of pretreatment methods on extraction and the extraction process to obtain antioxidant compounds from Ruellia tuberosa. The results show that the roots of Ruellia tuberosa contain a dry matter content of 69.22% and, a moisture content of 30.78%, and an ash content of 6.79%. In addition, the appropriate method of pre-treatment of raw materials is to dry the raw materials at a temperature of 60 °C in 4 h. Concentration of cellulase enzyme used 0.8% (v/w) and hydrolysis time is 60 minutes. The suitable extraction conditions were ethanol concentration of 50%, extraction time of 40 min at 50 °C gave polyphenol content of 10.20±0.28 mgGAE/g dry matter and flavonoid content of 4.05±0.24 mgQE/g dry matter. Research on extracting and collecting biologically active substances with antioxidant capacity from Ruellia tuberosa aims to consolidate and provide more valuable scientific information, thereby helping to exploit and use raw materials in practice more effectively.