Determination of cysteamine in animal feeds by high performance liquid chromatography with diode-array detection

  • Phuong Truc Nguyen Huynh
  • Phu Hoang Nguyen
  • Mai Anh Nguyen
Từ khóa: Animal feeds, Cysteamine, Ellman's reagent, HPLC-DAD

Tóm tắt

the determination of cysteamine supplementation in commercial animal feeds was developed.
Samples were extracted with a mixture of 0.5 % hydrochloric acid (HCl) – acetonitrile (ACN) (90:10,
v/v), matrix interferences were removed with a C18 cartridge, and cysteamine was derivatized using
5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) as Ellman's reagent targeting to the thiol group in the
molecule. Quantification of cysteamine was performed on a C18 column with DAD at 323 nm. The
developed method had a limit of detection (LOD) of 1.1 mg/l, good linearity of the calibration curve
(R2 0.9998), high recoveries (> 92 %), and high reproducibility (RSD < 2.0%).

điểm /   đánh giá
Phát hành ngày
2020-07-07
In ra
Tập. 21 Số. 2 (2018)
Chuyên mục
NATURAL SCIENCES - METHODOLOGIES