Xao Tam Phan (Paramignya trimera) methanol extract induced apoptosis in hepatocellular carcinoma HepG2 cell line in vitro

Tóm tắt

Introduction: Xao Tam Phan (Paramignya trimera) has long been used in Viet Nam as an herbal medicine for the treatment of Hepatitis, hepatocellular carcinoma, and diabetes. This study aimed to determine the anti-proliferation effect of Paramignyatrimera extract(P.trimera extract) on HepG2
hepatocellular carcinoma cells.

Methods: AlamarBlue assay was used to determine the IC50 values of P. trimera extract on HepG2 cells. Adipose-derived stem cells (ADSCs) was used as normal cell control. For apoptosis examination, P. trimera extract-treated HepG2 cells were incubated with Annexin V/Propidium iodide (PI). Then they have been analyzed their expression of Annexin-V and PI by flow cytometry. The cell nuclear degradation also was evaluated by PI/Hoechst 33342 staining assay.

Results: Doxorubicin and P. trimera extract IC50 values on HepG2 cells were 55.13 ± 2.028 ng/ml and 582.533 ± 16.521 µg/ml, respectively. Those on ADSCs were 5.96 ± 0.56 ng/ml and 268.976 ± 19.325 µg/ml, respectively. Side effect index value (SEI) of P. trimera extract was 2.175 ± 0.12, and the SEI of doxorubicin was 8.71 ± 0.36. Flow cytometry analysis indicated significant apoptosis on P. trimera extract-treated HepG2 cells at a dose of 500 µg/ml (32.39 ± 2.28%
apoptotic cells, and 14.63 ± 1.59% necrotic cells). Nuclear aggregation and degradation was seen on 500 µg/ml P. trimera treated HepG2 cells.

Conclusion: P. trimera extract could inhibit HepG2 hepatocellular carcinoma cell proliferation by inducing apoptosis