EFFECTIVE RECOVERY AND PURIFICATION OF POLY(3-HYDROXYBUTYRATE) FROM A HALOPHILIC BACTERIUM BY CHEMICAL DIGESTION METHOD

Tóm tắt

A simple and effective process for the recovery of intracellular
poly(3-hydroxybutyrate) (PHB) from a halophilic bacterial strain - Salinivibrio sp.
M318 was developed using a chemical digestion method. The effect of
temperature, chemicals (sodium hypochlorite and sodium hydroxide) and their
concentration on PHB recovery was examined. It was found that sodium hydroxide
was an effective chemical for the recovery of PHB from Salinivibrio sp. M318.
High PHB recovery yield of 97% and polymer purity of 99% were obtained when
50 g/L of bacterial cells were incubated in NaOH solution at the concentration of
0.075 M for 1 h at 50oC. It is expected that this simple method can be of interest
for other PHA production processes.