Study on synthesis of colloidal gold nanoparticles and preparation of E. coli O157 antibody conjugated nanoparticles
Abstract
The present paper describes the preparation of colloidal gold nanoparticles by a chemical reduction method, using some chemical compounds such as sodium citrate (Na3Ct), chloroauric acid (HAuCl4.3H2O) and anti-E. coli O157 monoclonal antibody-conjugated gold nanoparticles. The nanoparticles were characterized by several techniques including Ultraviolet and Visible Absorption Spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM). The UV-Vis confirmed that the concentration of Na3Ct has a major effect on the size of the synthesized gold nanoparticles, and TEM images showed that the size of gold particles is in the range of 10-20 nm. In addition, the binding of the antibody-gold conjugates to E. coli O157 was also investigated using the same characterization means.