Purification of pectinase from Asp.Niger by the techniques of gel filtration and cross-flow membrane

Abstract

Pectinase from Aspergillus niger by solid state medium containing powered carot as an inducer obtained the highest activity after 48 hours. Separating  enzyme on a gel filtration column packed with Bio gel P 30, which showed  that one peak contained  activity of pectinase, and with column flow rate of 15 ml/h the separation was better than 30 ml/h.

Using cross flow membrane with speed-pump of 150 rpm at the input, the purification of  enzyme (3.1 times before filtration) was  higher than at rate of 200rmp (2,2 times before filtration).