Synthesizing Biocoffee-1 and applying it in coffee fermentation

Abstract

In coffee processing today, attracting all the dissolved substances from the coffee bean is a matter that so many manufacturers are interested in. However, the dissolved substrate extraction is recognized by the two major components in the coffee bean: pectine and cellulose as from this research.  Pectine contains 52.62-55.14% and cellulose contains 15.29-17.04%. We have successfully created the bio-product named Biocoffee-1, which has high enzymatic activity of pectinase and cellulase. The optimal chemical components of culture for this bio-product synthesis includes carrot (9 %), rice husk (15%), mash (75%), NH₄(SO₄)₂  (1%) and other factors such as temperature 27-30⁰C (about room temperature), humidity 56-64%W, and time 40-44 hours. On a large scale, when the bio-product is needed for industrial production, it also needs to produce a larger quantity as well. We find that the wheat powder and cassava are the most appropriate for this process. We concluded that the ratio "wheat powder: cassava powder" is 2:1, humidity 56 %W and bio-synthesis time is optimal at 30 hours. Moreover, we also investigated the optimal fermented conditions for the highest quantity of dissolved substance weight. The dissolved substance weight is defined that the dry quantity of dissolved substance extracted from 10g of fermented coffee and dissolved rate is done by putting 10g of coffee powder in 50ml of hot water.  We concluded that coffee-bean humidity gets most after 3 hours in water. However; the highest quantity of dissolved substances weight was determined 1 hour after putting coffee in water, and the optimal fermented time was 16 hours and the ratio bio-product weight/ coffee weight is16/1000. Thus, this bio-product can solve the problem as early mentioned and get the high quality of coffee with the fermented method, a new way in coffee processing today.