Antimicrobial resistance among Campylobacter spp. Strains isolated from faeccal samples of pigs chickens and ducks at Dong Thap province.

Abstract

This study was designed to identify the genetic basis that results in the  development of antibiotic resistance  in Campylobacter spp. We carried out a molecular examination of 75 strains looking for resistance factors. These strains were isolated from faecal samples of pigs, chickens and ducks at Dong Thap province. There were 89.3 % strains which showed mutations on DNA gyrase-gyrA gene at the position 86 (C257T and T227G). There were also a further 32 % strains  that showed mutations on gene cmeR encoding the efflux CmeABC pump system. This  study has also shown that 29.3 % of Campylobacter spp. have mutations in fluoroquinolone (FQ) resistance on both genes gyrA and cmeR. In comparison with  Campylobacter jejuni with the mutation C257T (Thr-86-Ile), Campylobacter coli has another mutation at T227G (Thr-86-Met). This mutation,  encoding  FQ resistance, is common in both swine and poultry but has not yet been identified in Viet Nam. As well as FQ resistance, resistance to erythromycine (Ery) has been detected in C. coli, in  10 strains erythromycin resistance, with a mutation at  position A2075G. There was 1 strain which carried two mutations A2075G and A2074C at 23S-rRNA gene, and 2 mutant strains with a double mutation at A2075G and A2076C. In summary, there were three strains of C. coli with double mutations encoding Ery resistance  at  Dong Thap and this resistance characteristic has not  been identified eslewhere.