Production and characterization of sophorolipid produced by Candida bombicola from coconut oil

Abstract

The biosurfactants from microbial origin increasingly gained interests because of  their application in many field and excellent properties compared to surfactants from chemical origin, such as the higher biodegradability, lower toxicity and environmentally friendly. Sophorolipids, biosurfactants of glycolipid groups are produced through the fermentation by nonpathogenic yeasts such as Candida bombicola. In this study, we investigated the production, surveyed properties of sophorolipids through fermentation by C. bombicola from coconut oil. The results showed that the yield of sophorolipid obtained after 7 days of culture was 14.6 g/L, the surface tension was 40 mN/m. The obtained sophorolipid showed ability to be resistant to some bacteria such as E. coli, B. subtilis, P. aeruginosa, and S. aureus. Through DPPH experiment, sophorolipids showed the scavenging acitivity with IC₅₀ = 1.4063 mg/mL. These results showed that sophorolipids could be applied in cosmetics.