RESEARCH ON ISOLATION, INVITRO CULTURE OF MARINE MICROALGAE (Chaetoceros calcitrans Paulsen, 1905) AND IT’S APPLICATION AS FOOD FOR WHITE SHIRMP (Penaeus vannamei )

Abstract

In 1940, two cultivation methods for siliceous marine algae were developted in Japan. Fujinaga determined that Skeletonema costatum and Chaetoceros sp. Algae originally were the main food of larval shrimps from the phase of Zoea until the phase of Postlavae. In Viet Nam, by early 1970’s, the cultivation of high value aquatic products started to atract attention. In this context, many researchs have been focus on algae cultivation in order to find the suitable species for local condition. In this study, Chaetoceros calcitrans algae were massed on TT3 medium with cell density of 20.10⁴ cell.ml^-1, 40.10⁴cell.ml^-1, 60.10⁴ cell.ml^-1, 80.10⁴ cell.ml^-1. The optimal cell density was 60.10⁴ cell.ml^-1. Chaetoceros calcitrans algae was cultured on Liao medium, TT3 medium, f2 medium. Using Chaetoceros calcitrans algae as supplemental fresh food for white shrims larval from the phase of Nauplius to the phase of 3 Mysis improved the larvae quality and increased the survial  rate of larvae from 42 percentage to 76 percentage.