Isolation and selection of Lactobacillus spp. antagonistic to Vibrio parahaemolyticus causing the (Early Mortality Syndrome) shrimp disease in Soc Trang province

Abstract

Early mortality syndrome (EMS) caused by pathogenic Vibrio parahaemolyticus is one of the most major factors affecting the development of aquaculture. Using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and has lots of potential to control pathogenic bacteria. In this study, we isolated and screened total of 8 Lactobacillus strains from 30 mud, water and shrimp samples at shrimp ponds in Soc Trang province. All of them were be able resistant with Vibrio parahaemolyticus strains causing the EMS shrimp disease in vitro. In which, TA7L1 strain showed the strongest resistance and was identified as Lactobacillus plantarum by analysing 16S rDNA sequence and MALDI-TOF. TA7L1 strain was determined safety and has potential application in the production of biological products to prevent EMS shrimp disease.