Biological activities and micropropagation of non-vascular plants from Bidoup-Nui Ba National Park, Lam Dong

Abstract

At the present time, non-vascular plants (bryophytes) are less studied, especially in Vietnam. Therefore, this research focuses on studying biological activities of  some bryophytes species collected in Bidoup–Nui Ba National Park, such as antioxidative, antibacterial activity, acetylcholinesterase inhibitory activity and quantitative analysis of the major secondary metabolites. Moreover, one of the most potential species was micropropagated in vitro in order to get the initiative pharmaceutical materials. Among the six bryophyte species collected in Bidoup–Nui Ba National Park, three of them are mosses (Pyrrhobryum spiniforme, Bryum argenteum and Campylopus umbellatus) and three of them are liverworts (Lepidozia fauriana, Plagiochila trabeculata and Schistochila blumei). Our results showed that L. fauriana had the highest antioxidative activity and acetylcholinesterase inhibitory activity (IC₅₀ = 6.617 ± 0.080 mg/mL), S. blumei had the potent antibacterial activity. In vitro propagation results showed that silver moss could be micropropagated using spores in MS medium supplemented with 1 mg/L 2,4-D and 2 mg/L kinetin.