Optimization of cellulase production by Trichoderma Reesei on solid substrate medium

Abstract

Sugarcane baggase/wheat bran ratio, initial moisture content, concentration of basal medium and cultivation time were optimized in solid state fermentation (SSF) for the production of cellulase by an Trichoderma reesei VTT-D-80133 mutant using experiment optimization. Sugarcane baggase/wheat bran ratio (7/3), initial moisture content of 60%, 8 times concentration of basal medium and cultivation time of 7 days were optimum for cellulase production in SSF. The activity and productivity of cellullase obtained after 7 days of fermentation were 280,64 IU/g and 5 FPU/g of fermented medium; These activities are lower than 3,2 and 37 times compared with commercial cellulase (Amano T) of AMANO Company (Japan). Besides cellulase, fermented substrate contain activities of α-amylase 268,75 UI/g, protease 12,43 UI/g and xylanase 10073,25 BXU/g. The cellulase extract was shown to have some bands that their molecular weights are equivalent to commercial cellulase (Amano T) by sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE). At the filter paper activity of cellulase was 5 FPU/ml, the yield of enzymatic hydrolysis of paper waste was 21%. The sugars (23,62 mg/ml) converted from paper waste by cellulase could be used further in the fermentation of valuable products.