Research on biocompatibility of cancellous - derived acellular scaffold

Abstract

Xenogeneic cancellous bone is a abundant resource on bone tissue engineering. But xenograft can arise a serious of immune responses. Thus, we decellularize xenogeneic cancellous bone for obtaining a great number of acellular and low antigenic scaffolds. Porcine cancellous bone (PCB) (4x4x3,5 cm³) were decellularized by acetone or chloroform/methanol (1:1). Biocompatibility of acellular scaffold was evaluated by seeding umbilical blood cells (UBCs) into scaffold and transplanting acellular scaffold to xeno-recipient. The result shows that PCB-derived scaffold treated in two decellularizing solutions have no cell. After 3 days, UBCs adhered on surface of scaffold and proliferated contiously after 12 days. After 8 days, scaffold makes a weak imflammatory response. The intial results show that PCB-derived acellular scaffold can apply in bone tissue engineering.