Study on bar Gene transformation into Ganihot esculenta crantz by biolistic method

Abstract

Cassava (Manihot esculenta) is the fourth important crop of Vietnam, not only in agriculture but also in industry. The productivity is not so much because of difficulty in planting due to a long time of cultivation and the invasion of weeds. Therefore, it is extremely important to create a herbicide resistant cultivar. In this paper, we used biolistic transformation to transform the bar gene – PPT (Phosphinothricin) herbicide resistance gene – into in vitro cassava samples and selected the transgenic shoots resisting PPT. The result showed that in vitro early shoots had the highest transgenic ratio at 6 cm fire distance with 1000 μg tungsten and 1.5 μg DNA mix. The transgenic shoots had been proved to carry bar gene by PCR with its specific primers BAR3/BAR4.