Identification and optimization of protease production by Bacillus sp. B9 isolated from waste water
Tóm tắt
Proteases have high commercial value and find multiple applications in various industrial sectors in detergent, leather, textile, food and pharmaceutical industry. This study investigated the protease production and optimization by bacterial strain which named Bacillus sp. B9 isolated from waste water. The bacterial isolate was identified by morphological, biochemical and 16S rRNA phylogenetic analysis using 27F and 1492R primers has 99.64% sequence identity (100% query cover) with corresponding gene sequence of Bacillus horneckiae strain OOM25 (MH542294.1) on GenBank. The crude enzyme extract of the strain was also characterized the activity with respect to temperature, pH, incubation period and two different enzyme substrates. High protease activity produced by Bacillus sp. B9 was observed at 36h of incubation at 45oC with the initial pH of 9.0 by using gelatin as its substrate. Additionally, when using gelatin substrate, the protease activity was much higher than in the case of casein.