DEVELOPMENT OF IMMUNOCHROMATOGRAHY TEST KIT FOR SCREENING CLENBUTEROL IN FEED FOOD

Abstract

Clenbuterol  belongs  to  the  P2-agonist.  Some  stadies  reported  that  the  P2-agonist  including  Clenbuterol could promote muscle growth and reduce body fat. When animals are tteated with Pa-agonist, the accumulated residue  in their  tissue  may  have  a effect  in human.  This  article  reported  an  immunochromatography  device based on the principle of  inhibitor assay. The Clenbuterol in the feed food inhibited with the monocloanl anti-Clenbuterol antibody in the conjugate  pad. This antibody was not be captured by the Clenbuterol-BSA coated on  the  nittocellulose  membrane  for  the  limited  binding  sites,  so  T  line  disappear.  When  an  amount  of  the extracted  feed  food  was applied to the sample pad, the solution migrates by  capillary  action through  the test strip. If it was negative, the T line  appeared as a visible line. If it was possitive, no T line developed in the T region.   This  method  had  high  sensitivity  and  specificity.  Result  was  read  rapidly  in  serveral  minutes.  We chosen  several  appropriate  conditions  as:  concenttation  of  capture  reagents  on  test  line  and  control  line, concenttation  of colloidal  conjugated  gold on the conjugate  pad, treated  sample pad to make a rapid test. We

successfully  created a rapid test to detect Clenbuterol in feed  food.  The method and device have high exactly (97%)  and faul  positive  rate  (3.6%), limited  detection  Clenbuterol  in water  is 5ppb  and  feed  food  is  0,0625 mg/kg Reading results are set at 5 - 10 minutes. We also make a sample exttactor which is simple and activites in feld. Store the test below room temperature. Expiry date is about 18 months.