EVALUATION OF THE GENETIC DIVERSITY OF 19 MECHILIA MEDIOCRIS SAMPLES BY RAPD AND CHLOROPLAST DNA MARKERS

Abstract

To contribute to the development of an efective  sttategy for conservation of Mechilia mediocris, we used fifteen  random primers and seven chloroplast primers to analyse the genetic diversity of  19 Mechilia mediocris samples  that  were  collected  from  three  different  locations  (Phu  Tho,  Cue  Phuong  and  Sai  Gon).  All  of  15 RAPD  primers  gave  DNA  polymophism.  In  the  analysed  regions  of  0.25  -  3  kb,  there  were  134  DNA fragments  amplified and 104 DNA fragments were polymorphic. Genetic similarity coefficients  of  19 Mechilia mediocris  samples ranged  from  0.568  (CP2  and  SGS) to  0.950  (PT2 and PT3). The phylogenetic free of  19 Mechilia mediocris samples was divided into two main groups. The first group included only SG8 sample and the second  consisted  remained  samples which  were  classified  into  six  subgroups. According  to  the result  of RAPD-PCR  analyses,  the  samples  that  were  collected  from  the  same  geographical  region  pertained  to  one subgroup. When  we  stadied  genetic  diversity  of  19 Mechilia mediocris samples  using  7 chloroplast  primer, four primer pairs generated DNA bands from 1 to  1.25 kb in length. We used 14 restriction enzymes to analyse PCR products  of these primer pairs. However,  only  6 restriction  enzymes had  cleaved PCR fagments  but no polymorphism was observed. Taken together, our results proved that all  19 Mechilia mediocris samples belong to Mechilia mediocris species.