IMMOBILIZING SACCHAROMYCES CEREVISIAE N28 CELLS BY BACTERIAL CELLULOSE AND APPLICATION

Abstract

Bacterial cellulose (BC) has many good qualities: high purity, water absortion, plasticity. Be can be used as matrix absorbing cultural medium for the growth of microbial biomass. Our study focused on the improvement of immobilization of Saccharomyces cerevisiae N28 using BC and comparison with alginate. The BC showed 4 major advantages in comparison with Na-alginate in immobilization of S. cerevisiae N28: more stability in mechanical and physical properties, fermentation equipment, lower cost (6 fold) and ligher reutilization (2 - 3 fold). The granular A-BC matrix absorbed more culture medium, in turn more cells than surface BC from 0.3.10⁸ to 1,1.10⁸ cells/g. The best conditions for immobilizing S. cerevisiae N28 were 4 days entrapment, at 30°C and under shaking 250 rpm. The efficiency was very high the BC might be reused continuously for at least 8 fermentations