USING PIEZO FOR ENUCLEATION OF MOUSE OOCYTES IN SOMA TIC CELL NUCLEAR TRANSFER IN VIETNAM

Abstract

This study was performed for determination of suitable parameters of Piezo equipment during the enucleation of mouse oocyte prior to somatic cell nuclear transfer. Female mouse (8 - 12 week old) was superovulated by administration of PMSG (Pregnant Mare Serum Gonadotropin) JO I.U.lfemale (day I), hCG (Human Chorionic Gonadotropin) 5 I.U.lfemale (day 3) and collection of matured oocyte was carried out at about 13 - 16 hours after hCG injection. The good oocytes were collected and enucleation was conducted with the help of Piezo equipment. Results show that, the average number of superovulated oocyte was 25.22 ± 10,43/female and of good oocytes was 17.31 ± 9.40/female (65.73 ± 12.57%), efficiency of the enucleation reaches 80% with the help ofPiezo equipment (Brandwidth was 50 or 100 KHz and Frequency was 2 - 5 Hz, Duration was 66 - 75 flS, Amplitude was 10 - 30 V).