USE OF PCR-DGGE TECHNIQUE TO ANALYSE SULFATE-REDUCING BACTERIAL COMMUNITY STRUCTURE IN LAKE SEDIMENTS IN THE HERBICIDEIDIOXIN CONTAMINATED AREA AT DANANG AIRPORT

Abstract

At present, soil and lake sediments in some "hot spots" including US former military base in Danang were still heavily contaminated by herbicide/dioxin. Bioremediation technology was successfully applied for detoxification of polluted soil at Danang and has been potential to treat contaminated lake sediments. In general, anaerobic bacteria which include sulfate reducing bacteria play an important role in material transformation cycle and dechlorination in sediments. Culture based techniques usually enables isolation of a small fraction of microbial community. Now, rRNA dependent techniques including DGGE (denature gel gradient electrophoresis) are widely used in studies of microbial ecology. In this report, nested-PeR combinated DGGE were used to analyse sulfate reducing bacterial community in lake sediments at herbicide/dioxin contaminated area in Danang. There was no significant difference between contaminated and uncontaminated lake sediments and between lakes which are under direct and indirect influence from contaminated area. Sulfate reducing bacterial community was not changed so much during two sampling times. Some strong DNA bands appeared only in one or several sediments. Among 13 lake sediments, two A4 and AS samples showed the lowest diverse level of sulfate reducing bacteria.