Study on multiligand complex formation in the systems: methylmol blue - Ti(IV)-HX (HX: acids tartric, sunfosalicilic) by spectrophotometric method and analytical application possibility.

Abstract

Studies on the inhibition of curcumin from Curcuma tonga on phospholipase A₂ from cobra venom (Naja naja) were carried out, The phospholipase was fractionated by ion-exchange chromatography on a CM-cellnlose column (10 x 60 mm) following chromatography on a superdex G-75 column (16 x 90 mm) and its biochemical properties were studied accurately. The phospholipid's hydrolysis catalyzed by phospholipase A₂ has been inhibited by curcumine from Curcuma longa. If seems that, the phospholipase A₂ inhibition isfollowing tile purely competitive mechanism. The constant of phospholipide hydrolysis K₁ and inhibition cons/ant k2 have been calculated: K₁ = 5.8x10⁴ M, k2 = 0.21.10^-3.s^-1.