GENE EXPRESSION ANALYSIS INVOLVED IN FLAVONOID BIOSYNTHESIS OF PHYLLANTHUS AMARUS

Abstract

Flavonoids, a diverse group of secondary metabolites, are known for their health-promoting properties, including antioxidant, anti-inflammatory, and
anticancer activities. Phyllanthus amarus is a medicinal plant with extensive traditional use and it is a rich source of flavonoids. This study aimed to elucidate the
gene expression in flavonoid biosynthesis in P. amarus. Using quantitative polymerase chain reaction (qPCR), we investigated the expression of Phenylalanine
ammonia-lyase (PAL), chalcone synthase (CHS), and chalcone isomerase (CHI), which are key genes associated with flavonoid biosynthesis. Our findings
contribute to a deeper understanding of flavonoid biosynthesis in P. amarus and provide valuable insights into the molecular mechanisms involved. This
knowledge can be leveraged to optimize the production of specific flavonoids with potential health benefits and to develop strategies for enhancing the
bioactivity of P. amarus as a medicinal plant. This study thus contributes to the expanding field of plant biochemistry and lays the foundation for further research
on the biosynthesis and regulation of flavonoids in Phyllanthus amarus.