SECONDARY METABOLITES FROM ACTINOPLANES MISSOURIENSIS AND INHIBITORY ACTIVITY OF Mtb ClpC1 PROTEIN

Abstract

From the culture solution of Actinoplanes missouriensis, two compounds including flufuran (1) and trehalose (2) were isolated. The structures of these compounds
were determined based on the analysis of NMR, ESI-MS spectral data and comparison with references. Compound 1 was evaluated the ATPase hydrolysis activity of the
recombinant ClpC1 protein. The results showed that this compound has the ability to affect the hydrolysis of ClpC1, a regulatory protein in Clp protease that plays an
important role in degrading misfolded proteins of Mycobacterium tuberculosis.