STUDY OF HYDROLYSIS EFFICIENCY OF ISOFLAVONES FROM SOY GERM EXTRACTS BY CELLULASE ENZYME

Abstract

Soy germ is a rich source of isoflavones with many benefits for human health.
Isoflavones have anti-oxidant, anti-cancer, anti-microbial, and anti-inflammatory
properties, and improve female physiology. These benefits of isoflavones are
determined by the aglycone form while the aglycone isoflavone content is only
about 2 - 5% of the total isoflavones. This study deals with the use of cellulase to
hydrolyze glucoside isoflavones into aglycone forms. Choosing the appropriate
enzyme and regimen will help improve the hydrolysis efficiency of isoflavone
glucosides. The result showed that with cellulase enzyme at pH 5, temperature
50o
C, the content of enzyme is 1.5U/g for 5 hours, the efficiency of hydrolysis of β-
glucosides; malonyl glucosides, acetyl glucosides are 95.84%; 64.82; 89.11%,
respectively. Total algycones content at 5 hours was 35.91 ± 0.03µmol/g dried
weight (including 16.67 ± 0.02µmol daidzein; 5.70 ± 0.02µmol glycitein; 13.54
± 0.01µmol genistein).