DEVELOPMENT AND VALIDATION OF AN ASSAY METHOD FOR MAGNESIUM IN SOFT CAPSULES BY AAS

Tóm tắt

A procedure for quantitative analysis of trace element magnesium (Mg) in soft capsules (mainly
containing vitamins, trace elements and herbal extracts) using atomic absorption spectroscopy
(AAS) has been established.
The sample was treated to convert the Mg compound to Mg+2 ion form in solution by heating
the sample on a boiling water bath with 10% hydrochloric acid solution, with the addition of
Polysorbate 80. The sample was diluted with 1% hydrochloric acid solution, with the addition of
LaCl3 in the final solution for AAS measurement. The standard solutions were prepared by diluting
the 1000 µg/ml magnesium stock standard solution with 1% hydrochloric acid solution. Using an
atomic absorption spectrometer equipped with a Magnesium hollow cathode lamp and an acetyleneair flame, measure the atomic absorption of the standard and test solutions at the maximum spectral
line of Magnesium at 285.2 nm. Calculate the results using the standard curve method.
The method validation results showed that the method has high specificity, linear range from 0.1
µg/ml to 0.5 µg/ml with correlation coefficient R2 = 0.9972, high accuracy (from 99.19 -101.39%),
good repeatability with small CV% values (0.44 to 0.91%), the method's determination range is
from 0.18 to 0.32 µg/ml