STUDYING TO BUILD THE DETERMINATION PROCESS OF AMMELIDE (AMD) AND DICYANDIAMIDE (DCD) IN ANIMAL FEED BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) WITH A DIODE-ARRAY DETECTOR (DAD)

Tóm tắt

The purpose of this study is to built a process for determining the content of fake protein enhancers such as Ammelide (AMD) and Dicyandiamide (DCD) in animal feed in accordance with the sample preparation procedure combined with a solid phase extraction (SPE) purification process and a high-performance liquid chromatography (HPLC) with a diodearray detector (DAD). The content of AMD and DCD in animal feed samples were determined with quantitative limits of 0.1-1.000 ppm, respectively for both substances, meeting AOAC (Association of Official Analytical Chemists) requirements for method validation and requirements for sensitivity, repeatable, linear intervals to be practically applicable. The procedure of determination has been effectively applied at National Centre for Veterinary Drugs and Bio-Products Control No. 2 to control the content of AMD and DCD in the basis of actual animal feed samples.
Keywords: Fake protein enhancers, AMD, DCD, animal feed, SPE, HPLC-DAD.