THE EFFECTS OF ENZYME CELLULASE AND MICROWAVE ON FUCOIDAN EXTRACTION FROM Ceratophyllum demersum

Tóm tắt

Ceratophyllum demersum has many bioactive ingredients, notably fucoidan. Fucoidan is a fucose-rich polysaccharide often present in seaweed, having many functions related to physiological activities, demonstrated through biological activities such as anti-cancer, immune, anti-inflammatory or antioxidant, ect. Therefore, this compound is often used a lot in functional foods. This aimed study is conducted to determinate the effects of the solvent system of impurities, enzyme, microwave effect on fucoidan content extracted from C. demersum seaweed. The results showed that obtained fucoidan content in solvent system 1 was 794.894 ± 6.054 μg/gdm, higher than that of solvent system 2 (574.851 ± 5.569 μg/gdm). In addition, in microwave-assisted method, the fucoidan content was 986.968 ± 3.514 μg/gdm while the figure was 1049.046 ± 9.838 μg/gdm in enzyme-assisted method at 50 °C for 3 hours and the ratio of enzyme/ material 2/20 (v/w). Moreover, the the combination of enzyme and microwave in fucoidan extraction with the microwave-enzyme sequence resulted in fucoidan content of 1246.871 ± 8.352 μg/gdm, which is significantly higher than the reverse sequence investigated in this study.