ASSESSMENT OF PHYTOCHEMICAL COMPOSITION, ANTIMICROBIAL, ANTIOXIDANT ACTIVITIES, AND CYTOTOXICITY OF THE ETHANOL EXTRACT FROM CORDYCEPS MILITARIS

Tóm tắt

Cordyceps militaris (C. militaris) has been utilized as an herbal medicine with diverse therapeutic properties in Asian countries. It can be a prominent resource of secondary metabolites that possesses remarkable biological activities. Herein, this study investigated the phytochemical screening, antimicrobial, antioxidant, and cytotoxic activities of the fruiting bodies of C. militaris using ethanol solvent. Qualitative phytochemical screening of the ethanol extract revealed the presence of proteins, carbohydrates, phenolics, tannins, flavonoids, and saponins, especially cordycepin and adenosine. The agar well diffusion method demonstrated that the ethanol extract exhibited sensitive activity against Staphylococcus aureus (ZOI = 30.33 ± 2.08 mm) and moderate activities against at for Salmonella spp. (ZOI = 16.33 ± 1.52 mm), Escherichia coli (ZOI = 9.33 ± 1.15 mm) as well as Aspergillus niger (ZOI = 7.83 ± 0.76 mm) at 100 mg/mL concentration. The 2,2-diphenyl[1]1-picrylhydrazyl (DPPH) free radical scavenging activity with the half-maximal inhibitory concentration (IC50) of 217.85 ± 33.16 µg/mL was conducted for the extract at 500 µg/mL. The protective effect against oxidative damage to DNA induced by hydrogen peroxide and UV radiation was observed with the treatment. Moreover, the results assessed C. militaris extract inhibited the growth of the MDA-MB-231 cell line compared to control. At the same time, these treatments affected the increase of the cell proliferation of normal human fibroblast cell line at 134.85 ± 35.18%. The data in this work indicated that C. militaris extract had significant biological properties and could be applied to herbal supplements and medicines in the pharmaceutical industry.