OPTIMIZATION OF ENZYME-ASSISTED EXTRACTION OF FLAVONOID FROM Glinus oppositifolius

Tóm tắt

Glinus oppositifolius, a potential medicinal herb used in many countries around the world, contains lots of bioactive compounds. One of the essential ingredients was flavonoid, a group of natural compounds that have many beneficial effects on human health, such as antioxidant functions, antibacterial, anti-inflammatory, and anti-cancer. The independent variables, including enzyme concentration (10-50 UI/g), temperature (50-70 °C), and time (60- 120 min), were investigated. The flavonoid extraction conditions were optimized with the CCD (Central Composite Design) design by response surface method (RSM). The results indicated that the optimal extraction conditions were found to be enzyme concentration (24.12 UI/g), temperature (68 °C), and time (99.8 min). Under such conditions, the highest content of flavonoid is 26.13 ± 0.05 mg/g of dry matter. These results suggest that enzyme treatment could help extract valuable components such as flavonoids that hold good potential for use in the food, cosmetic and pharmaceutical industries.