Water-Pine (Glyptostrobus pensilis (Staunt) K.Koch) Conservation by In Vitro Grafting Technique

Abstract

      Water-pine shoots (Glyptostrobus pensilis) derived from in in vitro culture of 8mm in size were grafted (whip grafting) on epicotyls of 30 day-old Chinese Fir (Cunninghamia lanceolata) plantlets. The Chinese fir individuals were produced by sowing seeds on basalt and coconut fiber mix with 7:3 ratio and 70% moisture The mix was autoclaved at 121^oC, 1 atm for 2 hours to sterilize. This grafting method produced complete in vitro grafted plants. They survived and grew well after transplanting to the natural conditions. The results might contribute to research for conservation of a rare and endangered water-pine species.