Study on The Technological Condition for Submerged Fermenter Culture of Clitocybe Maxima Strain
Abstract
Clitocybe maxima is an edible mushroom with light brown color. Its fruiting body grows out as a stick, and then differentiates into a cap. Clitocybe maxima is also called bamboo shoot or long stem mushroom. The fruiting body contains plenty of amino acids and beneficial minerals esental for the human health. The present study investigated growth of C. maxima strain under liquid fermentation conditions. The experiment results indicated that the C. maxima mycelium grew best on medium containing 2 g yeast extract + 2 g pepton + 0.5 g MgSO4.7H2O + 15 g glucose + 1.5 mg thiamin + 1,000 ml distilled water. On this medium, C. maxima mycelium grew fast and mycelial dry weight attained 33.9 g/ 1,000 ml with highest number of mycelial pellet. Different oxygen supply significantly affected the morphology and mycelial biomass. The optimal concentration of oxygen is 0.75 liters/1 liter of medium/minute; the rate of original spawn of grade 1 for inoculation is 10% of the medium volume. The most suitable incubation time was 84-96 hours. The use of liquid spawn for mushroom cultivation can reduce cultivating cycle by 12 days.