Effects of Plant Growth Regulators and Micronutrients on In vitro Growth and Flowering in Roses

Abstract

   An in vitro flowering system has been considered as a convenient tool not only to study flowering mechanism but also to produce commercial in vitro flowers. This study evaluated effect of plant growth regulators (BA and TDZ), AgNO₃ and CoCl₂ on growth and in vitro flowering of three rose varieties (HV, HD, HT). The results showed that all tested compounds had different effects on plant growth but only TDZ and AgNO₃ were able to induce flowering. On MS medium containing 0.2 mg/l TDZ, only HV variety was induced flowering with the rate of 72.2%, while on medium supplemented 30 μM AgNO₃, after 21-25 days, all three varieties HT, HV and HD were induced flowering with the rates of 30%, 40% and 50%, respectively; the flowers lasted as long as 14-16 days.