The extraction procedure of a pluthiophenol-rich extract from the leaves of Pluchea indica L. collected in Hanoi

Abstract

In this study, we evaluated some conditions affecting the extraction of pluthiophenol rich in pluthiophenol from the leaves of Pluchea indica collected in Hanoi. The investigated factors included extraction method, extraction solvent, medicinal plant size, material mass/solvent volume ratio, extraction time, and number of extraction times. Additionally, several methods of refining and enriching pluthiophenol in the Pluchea indica extract were also investigated. The criteria used to evaluate and select extraction conditions were the pluthiophenol content by the HPLC method. The best results included using hot extraction at 80 ^oC, extraction solvent of 60% ethanol, material size of 1 mm, extraction three times and 2 hours each time, with material/solvent ratios of 1/8, 1/6, and 1/6 (g/mL). Refining and enriching the pluthiophenol component was done using a liquid-liquid distribution and column chromatography (Amberlite XAD4 polymer adsorbent). The obtained extract after refining had a pluthiophenol content of 6.12%, and the pluthiophenol recovery efficiency of the whole process was 79.74% on a laboratory scale (50 g). In the 1 kg scale extraction procedure, the obtained extract had a pluthiophenol content of 6.29%.