Evaluation of parameters affecting recombinant PETase and MHETase activity in PET degradation

  • To Lan Anh Institute of Materials, Biology and Environment/Academy of Military Science and Technology
  • Dinh Thi Hoa Institute of Materials, Biology and Environment/Academy of Military Science and Technology
  • Le Thi Thu Hong Institute of Biology, Vietnam Academy of Science and Technology
  • Pham Kien Cuong Institute of Materials Science, Vietnam Academy of Science and Technology
  • Le Huy Hoang Institute of Materials, Biology and Environment/Academy of Military Science and Technology
  • Le Duy Khanh Institute of Materials, Biology and Environment/Academy of Military Science and Technology
  • Dang Phuong Nam Institute of Materials, Biology and Environment/Academy of Military Science and Technology
  • Nguyen Thi Xuan Institute of Materials, Biology and Environment/Academy of Military Science and Technology
  • Duong Thi Thanh Loan Institute of Materials, Biology and Environment/Academy of Military Science and Technology
Keywords: PETase; MHETase; Polyethylene terephthalate (PET); Biodegradation; Synthetic polymer.

Abstract

PETase and MHETase are two enzymes catalyzing the degradation of polyethylene terephthalate (PET) into terephthalic acid (TPA) and ethylene glycol (EG), which are environmentally friendly and non-toxic. The application of these enzymes holds significant potential for treating plastic waste and advancing green technologies in environmental protection. In this study, several factors affecting enzyme activity, including buffer composition, temperature, and pH, were investigated. The optimal conditions for PETase and MHETase activity were identified as a temperature range of 30–40 °C, pH 8–9, and 100 mM sodium phosphate buffer. Under these conditions, the recombinant enzymes hydrolyzed commercial PET film within 7 days of treatment, resulting in a significant change in the PET surface morphology and thereby demonstrating the effectiveness of PETase and MHETase in PET film cleavage. These results highlight the potential of two recombinant enzymes for plastic waste treatment.

điểm /   đánh giá
Published
2025-12-25
Issue
Vol. 108 (2025)
Section
Chemistry, Biology & Environment