Selection the strain of Aspergillus sp for biosynthesis of glucose oxydase enzyme

Abstract

The  glucose oxydase enzyme (GOx) (EC 1.1.3.4) binds to beta-D-glucopyranose (a hemiacetal form of the six-cacbon sugar glucose) and aids in breaking the sugar down into its metabolites. GOx is a dimeric protein that catalyzes the oxidation of beta-D-glucose into D- glucono – 1,5- lactone (which then hydrolyzes to gluconic acid) using molecular oxygen and releasing hydrogen peroxide. GOx can be used in the removal of either glucose or oxygen fromfoodstuffs in order to improve their storage capability. Among the 22 strains of Aspergillus  in the National Food industrial Culture Collection o Microorganism of Food Industries Research Institute (FIRI), 6 strains coded as 9.4; 9.74; 1922F1; 1923F2; A1; A20 showed high glucose oxydase enzyme synthesis activity. The strain A niger 9.4 and A20 had the highest glucose oxydase enzyme synthesis activity were selected to test forming aflatoxin. Both of them were negative with aflatoxin B1, B2, G1 and G2 on the test medium. The strain A. niger 9.4 synthesized GOx enzyme with the highest content (32.9 U/g o the biomass) was selected for further study to product glucose oxydase enzyme.