Amplication of dna technique in diversity analysis of endangered rare red wood (Dalbergia cochinchinensis) germplasm in Vietnam
Vũ Thị Thu HIền
Đinh Thị Phòng
Abstract
Dalbergia cochinchinensis is a rare wood tree species at risk and threatened with extinction due to economic and trade value high. RAPD and ISSR techniques were used to study the genetic relationship of 35 DNA samples collected from JokDon National Park (Dak Lak province) and Kbang (Gia Lai province). A total of 51 primers were used (23 ISSR and 28 RAPD), there were 31/51 primers revealed polymorphic with PIC value varying from 0 (IS8, OPD03,...) to 0.423 (P63). Among 163 fragments were amplified, of which 99 were polymorphic (accounting for 60.74%). Genetic similarity coefficients between 35 D. cochinchinensis samples ranged from 0.655 (Dc5 and Dc30) to 0.942 (Dc10 and Dc11). The pattern of grouping in the dendrogram divided 35 D.cochinchinensis samples into 2 main groups and have the genetic variation coefficients about 5.1% (1 - 0.949) to 29.3% (1 - 0.707). The first group include the only Dc9 sample have the genetic variation coefficient about 29.3% (1 - 0.707). The second group included 34 samples remaining with the genetic variation coefficient about 5.1% (1 - 0.949) to 26.0% (1 - 0.740).