SEPARATION AND DETERMINATION OF URINE PROTEOME FROMPATIENTS WITH ACUTE RENAL ALLOGRAFT REJECTION
Abstract
The discovery of urinary biomarkers is a main topicin clinical medicine. The development of proteomics has rapidly changed the knowledge on urine protein composition and probably will modify it again. Two-dimensional electrophoresis (2-DE) coupled with mass spectrometry has represented for years the technique of choice for the analysis of urine proteins and it is time
to draw some conclusions. In this study, we focusedon major ethodological aspects related to urine sample collection, storage, purification and analysis by 2DE with mass spectrometry in patients with acute renal allograft rejection. The obtained results are considered as preconditions for the subsequent studies about urine proteome in patients with acute renal llograft rejection.