SEPARATION AND DETERMINATION OF URINE PROTEOME FROMPATIENTS WITH ACUTE RENAL ALLOGRAFT REJECTION

  • Phạm Đức Đan
  • Đỗ Hữu Chí
  • Hà Phan Hải An
  • Nguyễn Bích Nhi
  • Phan Văn Chi

Abstract

The discovery of urinary biomarkers is a main topicin clinical medicine. The development of proteomics has rapidly changed the knowledge on  urine protein composition and probably will modify it again. Two-dimensional electrophoresis (2-DE) coupled with mass spectrometry has represented for years the technique of choice for the analysis of urine proteins and it is time

to draw some conclusions. In this study, we focusedon major ethodological aspects related to urine sample collection, storage, purification and  analysis by 2DE with mass spectrometry in patients with acute renal allograft rejection. The obtained results are considered as preconditions for the subsequent studies about urine proteome in patients with acute renal llograft rejection. 

điểm /   đánh giá
Published
2014-11-05
Section
Articles