Studying on marine actinomycete strain HLD 3.16 from the coast of Vietnam producing antimicrobial compounds.
Abstract
Streptomyces autotrophicus. Strain HLD 3.16 can produce some enzyme such as amylase, cellulase, protease and exhibited both antibacterial activities against Bacillus subtilis ATCC 6633, Sarcina lutea M5, Bacillus cereus var. mycoides ATCC 11778, Escherichia coli ATCC 15224, Escherichia coli PA2, Alcaligenes faecallis, Salmonella typhy IFO14193, Pseudomonas auroginosa and antifungal activity against Candida albicans ATCC 12031, Aspergillus niger 114. For producing high antimicrobial compounds, the strain was grown in medium containing (g/l): soluble starch 15; glucose 2,5; pepton 4; (NH4)2SO4 2,5; CaCO3 2. The
favourable conditions for antimicrobial compound production by the strain HLD 3.16 was established as pH of 7.0 - 7.5, temperature of 30oC, precultured supplement of 4,0 % (v/v), volume of medium in shaking flask of 10 % (v/v) and harvested time at 108 hours of cultivation. The crude antibiotic extracted from culture broth using n- butanol and 2-butanon were absorbed at wavelengths of 220, 260 nm and 258 nm, respectively.